Charge-altering releasable transporters enable phenotypic manipulation of natural killer cells for cancer immunotherapy.

ABSTRACT: Chimeric antigen receptor (CAR) natural killer (NK) cells are an emerging cell therapy with promising results in oncology trials. However, primary human NK cells are difficult to transfect, hampering both mechanistic studies and clinical applications of NK cells. Currently, NK cell CAR modification relies on viral vectors or cell activation. The former raises cost and tolerability issues, while the latter alters NK cell biology. Here, we report that readily synthesized and inexpensive nonviral charge-altering releasable transporters (CARTs) efficiently transfect primary human NK cells with messenger RNA without relying on NK cell activation. Compared with electroporation, CARTs transfect NK cells more efficiently, better preserve cell viability, and cause minimal reconfiguration of NK cell

SUBMITTER: Wilk AJ 

PROVIDER: S-EPMC7479957 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature