Adaptable haemodynamic endothelial cells for organogenesis and tumorigenesis.

ABSTRACT: Endothelial cells adopt tissue-specific characteristics to instruct organ development and regeneration^1,2. This adaptability is lost in cultured adult endothelial cells, which do not vascularize tissues in an organotypic manner. Here, we show that transient reactivation of the embryonic-restricted ETS variant transcription factor 2 (ETV2)³ in mature human endothelial cells cultured in a serum-free three-dimensional matrix composed of a mixture of laminin, entactin and type-IV collagen (LEC matrix) 'resets' these endothelial cells to adaptable, vasculogenic cells, which form perfusable and plastic vascular plexi. Through chromatin remodelling, ETV2 induces tubulogenic pathways, including the activation of RAP1, which promotes the formation of durable lumens^4,5

SUBMITTER: Palikuqi B 

PROVIDER: S-EPMC7480005 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature