An In Vitro Model of Nonattached Biofilm-Like Bacterial Aggregates Based on Magnetic Levitation.

ABSTRACT: Chronic infections are associated with the formation of nonattached biofilm-like aggregates. In vitro models of surface-attached biofilms do not always accurately mimic these processes. Here, we tested a new approach to create in vitro nonattached bacterial aggregates using the principle of magnetic levitation of biological objects placed into a magnetic field gradient. Bacteria grown under magnetic levitation conditions formed nonattached aggregates that were studied with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) and characterized quantitatively. Nonattached aggregates consisted of bacteria submerged into an extracellular matrix and demonstrated features characteristic of biofilms, such as a polymeric matrix that binds Ruby Red and Cong

SUBMITTER: Domnin P 

PROVIDER: S-EPMC7480373 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature