A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity.

ABSTRACT: Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence in biofluids can be indicative for (patho)physiological conditions. Studies aiming to resolve functionalities of EV or to discover EV-associated biomarkers for disease in liquid biopsies are hampered by limitations of current protocols to isolate EV from biofluids or cell culture medium. EV isolation is complicated by the >10⁵-fold numerical excess of other types of particles, including lipoproteins and protein complexes. In addition to persisting contaminants, currently available EV isolation methods may suffer from inefficient EV recovery, bias for EV subtypes, interference with the integrity of EV membranes, and loss of EV functionality. In

SUBMITTER: Zhang X 

PROVIDER: S-EPMC7480457 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature