Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer.

ABSTRACT: Circulating extracellular vesicles (EVs) were recognized as a promising source of diagnostic biomarker. However, there are limited studies published in this area, partly due to the limited number of detection platforms capable of detecting extracellular vesicles. In this study, extracellular vesicle immunoassays were developed using the Single Molecule array technology (SiMoa) and their clinical applications to cancer diagnosis were evaluated. Two extracellular vesicle detection assays, CD9-CD63 and Epcam-CD63, were designed to detect universal extracellular vesicles and tumour-derived extracellular vesicles, respectively. Our results show that CD9-CD63 and Epcam-CD63 SiMoa assays specifically detect extracellular vesicles but not free proteins with high sensitivities. The Epcam-CD63 level

SUBMITTER: Wei P 

PROVIDER: S-EPMC7480466 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature