The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles.

ABSTRACT: The transfer of microRNAs (miRs) via extracellular vesicles (EVs) is a functionally relevant mechanism of intercellular communication that regulates both organ homoeostasis and disease development. Little is known about the packaging of miRs into EVs. Previous studies have shown that certain miRs are exported by RNA-binding proteins into small EVs, while for other miRs and for large EVs, in general, the export mechanisms remain unclear. Therefore, a proteomic analysis of endothelial cell-derived large EVs was performed, which revealed that heterogeneous nuclear ribonucleoprotein U (hnRNPU) is abundantly present in EVs. EVs were characterized by electron microscopy, immunoblotting and nanoparticle tracking analysis. Taqman microRNA array and single qPCR experiments identified specific miR p

SUBMITTER: Zietzer A 

PROVIDER: S-EPMC7480565 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature