Calcium Dynamics in Astrocytes During Cell Injury.

ABSTRACT: The changes in intracellular calcium concentration ([Ca²⁺]) following laser-induced cell injury in nearby cells were studied in primary mouse astrocytes selectively expressing the Ca²⁺ sensitive GFAP-Cre Salsa6f fluorescent tandem protein, in an Ast1 astrocyte cell line, and in primary mouse astrocytes loaded with Fluo4. Astrocytes in these three systems exhibit distinct changes in [Ca²⁺] following induced death of nearby cells. Changes in [Ca²⁺] appear to result from release of Ca²⁺ from intracellular organelles, as opposed to influx from the external medium. Salsa6f expressing astrocytes displayed dynamic Ca²⁺ changes throughout the phagocytic response, including lamellae protrusion, cytosolic signaling during vesicle form

SUBMITTER: Wakida NM 

PROVIDER: S-EPMC7481337 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature