Histone deacetylase 5 regulates interleukin 6 secretion and insulin action in skeletal muscle.

ABSTRACT:

Objective

Physical exercise training is associated with increased glucose uptake in skeletal muscle and improved glycemic control. HDAC5, a class IIa histone deacetylase, has been shown to regulate transcription of the insulin-responsive glucose transporter GLUT4 in cultured muscle cells. In this study, we analyzed the contribution of HDAC5 to the transcriptional network in muscle and the beneficial effect of muscle contraction and regular exercise on glucose metabolism.

Methods

HDAC5 knockout mice (KO) and wild-type (WT) littermates were trained for 8 weeks on treadmills, metabolically phenotyped, and compared to sedentary controls. Hdac5-deficient skeletal muscle and cultured Hdac5-knockdown (KD) C2C12 myotubes were utilized for studies of gene expression and glucose metab

SUBMITTER: Klymenko O 

PROVIDER: S-EPMC7481569 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature