Microsieves for the detection of circulating tumor cells in leukapheresis product in non-small cell lung cancer patients.

ABSTRACT:

Background

Circulating tumor cells (CTC) in non-small cell lung cancer (NSCLC) patients are a prognostic and possible therapeutic marker, but have a low frequency of appearance. Diagnostic leukapheresis (DLA) concentrates CTC and mononuclear cells from the blood. We evaluated a protocol using two VyCAP microsieves to filter DLA product of NSCLC patients and enumerate CTC, compared with CellSearch as a gold standard.

Methods

DLA was performed in NSCLC patients before starting treatment. DLA product equaling 2×10⁸ leukocytes was diluted to 9 mL with CellSearch dilution buffer in a Transfix CTC tube. Within 72 hours the sample was filtered with a 7 µm pore microsieve and subsequently over a 5µm pore microsieve. CTC were defined as nucleated cells which stained for cy

SUBMITTER: Tamminga M 

PROVIDER: S-EPMC7481649 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature