Combining derivative and synchronous approaches for simultaneous spectrofluorimetric determination of terbinafine and itraconazole.

ABSTRACT: In this study, determination of terbinafine and itraconazole down to biological concentration level has been carried out. The determination is based on increasing the selectivity of the spectrofluorimetric technique by combining both derivative and synchronous spectrofluorometric approaches, which permits successful estimation of terbinafine at 257 nm and itraconazole at 319 nm in the presence of each other at Δλ of 60 nm. International Conference on Harmonization validation guidelines were followed to fully validate the method, and linearity was obtained for the two drugs over the range of 0.1-0.7 µg ml^-1 for terbinafine and 0.5-4.0 µg ml^-1 for itraconazole. Application of the method was successfully carried out in the commercial tablets with good agreement wi

SUBMITTER: Elmansi H 

PROVIDER: S-EPMC7481716 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature