Assessment of DNA Repair Gene Expressions in Vitrified Mouse Preantral Follicles.

ABSTRACT:

Objective

Vitrification of the ovarian tissue is one of the techniques recommended for preserving the fertility of women who are dealing with infertility. Despite its benefits, our information about the molecular aspects of ovarian follicles vitrification is somehow ambiguous. Therefore, the aim of this study was to evaluate the expression pattern of DNA repair genes in vitrified preantral follicles.

Materials and methods

In this experimental study, the isolated preantral follicles (n=906) from 14-16 days old mice (n=12) were divided into three groups: fresh, toxic and vitrified which were cultured in vitro for 12 days. Preantral follicles were vitrified using cryotop followed by exposure to equilibration solution for five minutes and vitrification solution (VS) for 3

SUBMITTER: Khodavandpour Z 

PROVIDER: S-EPMC7481908 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature