Repetitive Elements Contribute to the Diversity and Evolution of Centromeres in the Fungal Genus Verticillium.

ABSTRACT: Centromeres are chromosomal regions that are crucial for chromosome segregation during mitosis and meiosis, and failed centromere formation can contribute to chromosomal anomalies. Despite this conserved function, centromeres differ significantly between and even within species. Thus far, systematic studies into the organization and evolution of fungal centromeres remain scarce. In this study, we identified the centromeres in each of the 10 species of the fungal genus Verticillium and characterized their organization and evolution. Chromatin immunoprecipitation of the centromere-specific histone CenH3 (ChIP-seq) and chromatin conformation capture (Hi-C) followed by high-throughput sequencing identified eight conserved, large (∼150-kb), AT-, and repeat-rich regional centromeres that

SUBMITTER: Seidl MF 

PROVIDER: S-EPMC7482064 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature