Creating Complex Polyacrylamide Hydrogel Structures Using 3D Printing with Applications to Mechanobiology.

ABSTRACT: Due to its favorable physical and chemical properties, including chemical inertness, low fouling by biological molecules, high porosity and permeability, optical transparency, and adjustable elasticity, polyacrylamide has found a wide range of biomedical and non-biomedical applications. To further increase its versatility, this communication describes a simple method, using readily available reagents and equipment, for 3D printing polyacrylamide hydrogels at a resolution of 100-150 μm to create complex structures. As a demonstration of the application, the method is used for creating a lab-on-a-chip cell culture surface with micropatterned stiffness, which then leads to the discovery of stiffness-guided collective cell segregation distinct from durotaxis. The present technology is expected

SUBMITTER: Wang YL 

PROVIDER: S-EPMC7482135 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature