Combining Mass Spectrometry with Paterno-Buchi Reaction to Determine Double-Bond Positions in Lipids at the Single-Cell Level.

ABSTRACT: Single cell MS (SCMS) techniques are under rapid development for molecular analysis of individual cells among heterogeneous populations. Lipids are basic cellular constituents playing essential functions in energy storage and the cellular signaling processes of cells. Unsaturated lipids are characterized with one or multiple carbon-carbon double (C═C) bonds, and they are critical for cell functions and human diseases. Characterizing unsaturated lipids in single cells allows for better understanding of metabolomic biomarkers and therapeutic targets of rare cells (e.g., cancer stem cells); however, these studies remain challenging. We developed a new technique using a micropipette needle, in which Paternò-Büchi (PB) reactions at C═C bond can be induced, to determine locations of C═C bonds in

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC7482314 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature