Single-cell RNA-Seq reveals the transcriptional landscape and heterogeneity of skin macrophages in Vsir^-/- murine psoriasis.

ABSTRACT: Rationale: V-domain immunoglobulin suppressor of T cell activation (VISTA) is a novel inhibitory immune checkpoint molecule. Vsir^-/- mice have exacerbated psoriasis-like skin inflammation. The immune cell subsets involved in inflammation in Vsir^-/- psoriatic mice are largely unknown. We have used scRNA-seq as an unbiased profiling strategy to study the heterogeneity of immune cells at a single cell level in the skin of Vsir^-/- psoriatic mice. Methods: In the present study, the right ear and shaved back skin of wild type and Vsir^-/- mice were treated with IMQ for 5 consecutive days to induce psoriasis-like dermatitis. Then, the single-cell RNA sequencing analysis of mouse back skin lesions was performed using 10 × Genomics technique.

SUBMITTER: Qie C 

PROVIDER: S-EPMC7482809 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature