High accuracy gene expression profiling of sorted cell subpopulations from breast cancer PDX model tissue.

ABSTRACT: Intratumor Heterogeneity (ITH) is a functionally important property of tumor tissue and may be involved in drug resistance mechanisms. Although descriptions of ITH can be traced back to very early reports about cancer tissue, mechanistic investigations are still limited by the precision of analysis methods and access to relevant tissue sources. PDX models have provided a reproducible source of tissue with at least a partial representation of naturally occurring ITH. We investigated the properties of phenotypically distinct cell populations by Fluorescence activated cell sorting (FACS) tissue derived cells from multiple tumors from a triple negative breast cancer patient derived xenograft (PDX) model. We subsequently subjected each population to in depth gene expression analysis. Our findin

SUBMITTER: Porter W 

PROVIDER: S-EPMC7482927 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature