Prediction of TP53 mutations by p53 immunohistochemistry and their prognostic significance in gastric cancer.

ABSTRACT:

Background

Recently, molecular classifications of gastric cancer (GC) have been proposed that include TP53 mutations and their functional activity. We aimed to demonstrate the correlation between p53 immunohistochemistry (IHC) and TP53 mutations as well as their clinicopathological significance in GC.

Methods

Deep targeted sequencing was performed using surgical or biopsy specimens from 120 patients with GC. IHC for p53 was performed and interpreted as strong, weak, or negative expression. In 18 cases (15.0%) with discrepant TP53 mutation and p53 IHC results, p53 IHC was repeated.

Results

Strong expression of p53 was associated with TP53 missense mutations, negative expression with other types of mutations, and weak expression with wild-type TP53 (p<.001). The sensiti

SUBMITTER: Hwang HJ 

PROVIDER: S-EPMC7483024 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature