A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.).

ABSTRACT: Dwarf architecture is an important trait associated with plant yield, lodging resistance and labor cost. Here, we aimed to identify a gene causing dwarfism in watermelon. The 'w106' (dwarf) and 'Charleston Gray' (vine) were used as parents to construct F₁ and F₂ progeny. Dwarf architecture of 'w106' was mainly caused by longitudinal cell length reduction and was controlled by a single recessive gene. Whole-genome sequencing of two parents and two bulk DNAs of F₂ population localized this gene to a 2.63-Mb region on chromosome 9; this was further narrowed to a 541-kb region. Within this region, Cla015407, encoding a gibberellin 3β-hydroxylase (GA3ox), was the candidate gene. Cla015407 had a SNP mutation (G → A) in the splice acceptor site of the intron, lead

SUBMITTER: Sun Y 

PROVIDER: S-EPMC7483442 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature