Proteomics provides insights into the inhibition of Chinese hamster V79 cell proliferation in the deep underground environment.

ABSTRACT: As resources in the shallow depths of the earth exhausted, people will spend extended periods of time in the deep underground space. However, little is known about the deep underground environment affecting the health of organisms. Hence, we established both deep underground laboratory (DUGL) and above ground laboratory (AGL) to investigate the effect of environmental factors on organisms. Six environmental parameters were monitored in the DUGL and AGL. Growth curves were recorded and tandem mass tag (TMT) proteomics analysis were performed to explore the proliferative ability and differentially abundant proteins (DAPs) in V79 cells (a cell line widely used in biological study in DUGLs) cultured in the DUGL and AGL. Parallel Reaction Monitoring was conducted to verify the TMT results. γ ra

SUBMITTER: Liu J 

PROVIDER: S-EPMC7483447 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature