Automation and low-cost proteomics for characterization of the protein corona: experimental methods for big data.

ABSTRACT: Nanoparticles used in biological settings are exposed to proteins that adsorb on the surface forming a protein corona. These adsorbed proteins dictate the subsequent cellular response. A major challenge has been predicting what proteins will adsorb on a given nanoparticle surface. Instead, each new nanoparticle and nanoparticle modification must be tested experimentally to determine what proteins adsorb on the surface. We propose that any future predictive ability will depend on large datasets of protein-nanoparticle interactions. As a first step towards this goal, we have developed an automated workflow using a liquid handling robot to form and isolate protein coronas. As this workflow depends on magnetic separation steps, we test the ability to embed magnetic nanoparticles within a prote

SUBMITTER: Poulsen KM 

PROVIDER: S-EPMC7483600 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature