Longitudinal proteomics analysis in the immediate microenvironment of islet allografts during progression of rejection.

ABSTRACT: The applicability and benefits of pancreatic islet transplantation are limited due to various issues including the need to avoid immune-mediated rejection. Here, we used our experimental platform of allogeneic islet transplant in the anterior chamber of the eye (ACE-platform) to longitudinally monitor the progress of rejection in mice and obtain aqueous humor samples representative of the microenvironment of the graft for accurately-timed proteomic analyses. LC-MS/MS-based proteomics performed on such mass-limited samples (~5 μL) identified a total of 1296 proteins. Various analyses revealed distinct protein patterns associated with the mounting of the inflammatory and immune responses and their evolution with the progression of the rejection. Pathway analyses indicated predominant changes

SUBMITTER: Alcazar O 

PROVIDER: S-EPMC7483621 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature