Loss of Uncoupling Protein 3 Attenuates Western Diet-Induced Obesity, Systemic Inflammation, and Insulin Resistance in Rats.

ABSTRACT:

Objective

Uncoupling protein 3 (UCP3) is a mitochondrial carrier related to fatty acid metabolism. Although gene variants of UCP3 are associated with human obesity, their contribution to increased adiposity remains unclear. This study investigated the impact that loss of UCP3 has on diet-induced obesity in rats.

Methods

Male UCP3 knockout rats (ucp3^-/- ) and wild-type littermates (ucp3^+/+ ) were fed a high-fat, high-carbohydrate Western diet for 21 weeks. Body composition was analyzed by EchoMRI. Whole-body insulin sensitivity and rates of tissue glucose uptake were determined by using hyperinsulinemic-euglycemic clamp. Changes in tissue physiology were interrogated by microscopy and RNA sequencing.

Results

Loss of UCP3 decreased fat mass gain, w

SUBMITTER: Lomax TM 

PROVIDER: S-EPMC7483834 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature