Functional Genetic Screening Enables Theranostic Molecular Imaging in Cancer.

ABSTRACT:

Purpose

Targeted therapies for cancer have accelerated the need for functional imaging strategies that inform therapeutic efficacy. This study assesses the potential of functional genetic screening to integrate therapeutic target identification with imaging probe selection through a proof-of-principle characterization of a therapy-probe pair using dynamic nuclear polarization (DNP)-enhanced magnetic resonance spectroscopic imaging (MRSI).

Experimental design

CRISPR-negative selection screens from a public dataset were used to identify the relative dependence of 625 cancer cell lines on 18,333 genes. Follow-up screening was performed in hepatocellular carcinoma with a focused CRISPR library targeting imaging-related genes. Hyperpolarized [1-¹³C]-pyruvate was inject

SUBMITTER: Perkons NR 

PROVIDER: S-EPMC7483881 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature