Functionally uncoupled transcription-translation in Bacillus subtilis.

ABSTRACT: Tight coupling of transcription and translation is considered a defining feature of bacterial gene expression^1,2. The pioneering ribosome can both physically associate and kinetically coordinate with RNA polymerase (RNAP)^3-11, forming a signal-integration hub for co-transcriptional regulation that includes translation-based attenuation^12,13 and RNA quality control². However, it remains unclear whether transcription-translation coupling-together with its broad functional consequences-is indeed a fundamental characteristic of bacteria other than Escherichia coli. Here we show that RNAPs outpace pioneering ribosomes in the Gram-positive model bacterium Bacillus subtilis, and that this 'runaway transcription' creates alternative rules for both global

SUBMITTER: Johnson GE 

PROVIDER: S-EPMC7483943 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature