Structural Visualization of Septum Formation in Staphylococcus warneri Using Atomic Force Microscopy.

ABSTRACT: Cell division of Staphylococcus adopts a "popping" mechanism that mediates extremely rapid separation of the septum. Elucidating the structure of the septum is crucial for understanding this exceptional bacterial cell division mechanism. Here, the septum structure of Staphylococcus warneri was extensively characterized using high-speed time-lapse confocal microscopy, atomic force microscopy, and electron microscopy. The cells of S. warneri divide in a fast popping manner on a millisecond timescale. Our results show that the septum is composed of two separable layers, providing a structural basis for the ultrafast daughter cell separation. The septum is formed progressively toward the center with nonuniform thickness of the septal disk in radial directions. The peptidoglycan on the inner su

SUBMITTER: Su HN 

PROVIDER: S-EPMC7484183 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature