Urinary Amine Metabolomics Characterization with Custom 12-Plex Isobaric DiLeu Labeling.

ABSTRACT: Lower urinary tract symptoms (LUTS) is common in aging males. Disease etiology is largely unknown but likely includes inflammation and age-related changes in steroid hormones. Diagnosis is currently based on subjective symptom scores, and mainstay treatments can be ineffective and bothersome. Biomarker discovery efforts could facilitate objective diagnostic criteria for personalized medicine and new potential druggable pathways. To identify urine metabolite markers specific to hormone-induced bladder outlet obstruction, we applied our custom synthesized multiplex isobaric tags to monitor the development of bladder outlet obstruction across time in an experimental mouse model of LUTS. Mouse urine samples were collected before treatment and after 2, 4, and 8 weeks of steroid hormone treatmen

SUBMITTER: Wei P 

PROVIDER: S-EPMC7484200 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature