Synthesis of a far-red emitting flavonoid-based lysosome marker for live cell imaging applications.

ABSTRACT: A bright far-red emitting flavonoid derivative (FuraET) was synthesized in good yields by inserting a π extension group (i.e., furan) into the flavonoid skeleton, via using the Suzuki-Miyaura cross-coupling reaction. FuaraET exhibited optical absorption at λ_ab ≈ 450 nm and emission λ_em ≈ 660 nm by recognizing as the first far-red emitting flavonoid derivative reported. FuraET exhibited a large Stokes shift (Δλ > 150 nm) high fluorescent quantum yield (φ_fl ≈ 0.2-0.4), and good photostability indicating excellent characteristics for an imaging probe. Live cell fluorescent confocal microscopy imaging revealed the exceptional selectivity of the FuraET towards cellular lysosomes (Mander's overlap coefficients >0.9). The observed non-alkalinizing nature and high

SUBMITTER: Bertman KA 

PROVIDER: S-EPMC7484329 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature