A biocomposite-based rapid sampling assay for circulating cell-free DNA in liquid biopsy samples from human cancers.

ABSTRACT: Cell-free nucleic acids (cfNAs) in liquid biopsy samples are emerging as important biomarkers for cancer diagnosis and monitoring, and for predicting treatment outcomes. Many cfNA isolation methods have been developed recently. However, most of these techniques are time-consuming, complex, require large equipment, and yield low-purity cfNAs because the genetic background of normal cells is amplified during cell lysis, which limits their clinical application. Here, we report a rapid and simple cfNA sampling platform that can overcome the limitations of conventional methods. We synthesised a biocomposite by combining amine-modified diatomaceous earth (DE) and cucurbituril (CB). The biocomposite platform showed high capture efficiency (86.78-90.26%) with genomic DNA and amplified DNA products

SUBMITTER: Koo B 

PROVIDER: S-EPMC7484795 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature