Optimization of Sorghum Kafirin Extraction Conditions and Identification of Potential Bioactive Peptides.

ABSTRACT: The interest in extracting kafirins (KAF), the main storage protein from sorghum grain has recently increased due to its gluten-free content and the significant scientific evidence showing the health benefits of the bioactive peptides from cereal grains in human diets. The objectives were to obtain the highest percentage of KAF extraction using amyloglucosidase as pretreatment to increase the extraction yield and predict the bioactive peptides in the KAF. In this study, pretreatments with amyloglucosidase increased the extraction yield of KAF compared with extraction methods using only ethanol and sodium metabisulfite. Two protein fragment sequences were identified from KAF extract and were evaluated for potential bioactive peptide using the BIOPEP-UWM database, which suggest that KAF prot

SUBMITTER: Castro-Jacome TP 

PROVIDER: S-EPMC7484892 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature