The effect of polar headgroups and spacer length on the DNA transfection of cholesterol-based cationic lipids.

ABSTRACT: This article is related to the effects of the headgroups and spacer length of cationic lipids on transfection efficiency. To develop highly potent cationic lipids, a series of divalent lysine-diamine conjugated cholesterol-based cationic lipids with three different headgroups (ammonium, trimethyl ammonium, and guanidinium) were synthesized. The newly synthesized cationic lipids (1-6)A formed cationic liposomes in the presence and absence of a zwitterionic helper lipid, DOPE (dioleoylphosphatidylethanolamine). A gel retardation assay showed that most of the prepared lipoplexes could retard DNA migration in the presence of DOPE. We attempted to modify the diverse cationic headgroups to improve the transfection efficiency. However, the lysine-1,3-diaminopropane-conjugated choles

SUBMITTER: Radchatawedchakoon W 

PROVIDER: S-EPMC7484938 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature