Phosphorylation of <i>Toxoplasma gondii</i> Secreted Proteins during Acute and Chronic Stages of Infection.

ABSTRACT: The intracellular parasite Toxoplasma gondii resides within a membrane-bound parasitophorous vacuole (PV) and secretes an array of proteins to establish this replicative niche. It has been shown previously that Toxoplasma secretes kinases and that numerous proteins are phosphorylated after secretion. Here, we assess the role of the phosphorylation of strand-forming protein 1 (SFP1) and the related protein GRA29, two secreted proteins with unknown function. We show that both proteins form stranded structures in the PV that are independent of the previously described intravacuolar network or actin. SFP1 and GRA29 can each form these structures independently of other Toxoplasma secreted proteins, although GRA29 appears to regulate SFP1 strands. We show that an unstructure

SUBMITTER: Young JC 

PROVIDER: S-EPMC7485689 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature