Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis.

ABSTRACT:

Background

Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs) in CF lungs. We aimed to investigate changes in and associations between PMNs, glucose and L-lactate in sputum during antibiotic treatment. In addition, the effect of hemoglobin A1c and plasma glucose on these biomarkers were investigated.

Methods

We sampled non-induced sputum at day 0, 7, 14 and 42 in 27 chronically infected CF patients electively treated with 14 days of intravenous antibiotic. To analyze sputum samples, we used flowcytometry to count PMNs and colorimetric assays to estimat

SUBMITTER: Nielsen BU 

PROVIDER: S-EPMC7485830 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature