General Method for Peptide Recognition in Water through Bioinspired Complementarity.

ABSTRACT: A general method for peptide recognition has been elusive despite decades of research. Strong binding and selectivity among closely related peptides are necessary for biological applications but have been difficult to achieve with synthetic receptors. With inspiration from highly specific protein-protein and protein-ligand interactions, protein-sized, water-soluble imprinted nanoparticles were prepared via templated polymerization of peptides within cross-linked micelles. Combination of hydrophobic and polar interactions afforded micromolar to submicromolar binding affinities for selected tripeptides. A "golden pair" of functional monomers was identified to enhance both the affinity and selectivity of binding, and enabled differentiation of subtly different sequences including single-point

SUBMITTER: Fa S 

PROVIDER: S-EPMC7486028 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature