PiQSARS: A pipeline for quantitative and statistical analyses of ratiometric fluorescent biosensors.

ABSTRACT: Genetically encoded ratiometric fluorescent probes are cutting-edge tools in biology. They allow precise and dynamic measurement of various physiological parameters within cell compartments. Because data extraction and analysis are time consuming and may lead to inconsistencies between results, we describe here a standardized pipeline for•Semi-automated treatment of time-lapse fluorescence microscopy images.•Quantification of individual cell signal.•Statistical analysis of the data.First, a dedicated macro was developed using the FIJI software to reproducibly quantify the fluorescence ratio as a function of time. Raw data are then exported and analyzed using R and MATLAB softwares. Calculation and statistical analysis of selected graphic parameters are performed. In addition, a functional

SUBMITTER: Levy E 

PROVIDER: S-EPMC7486618 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature