Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov state models.

ABSTRACT: DNA glycosylase is responsible for repairing DNA damage to maintain the genome stability and integrity. However, how glycosylase can efficiently and accurately recognize DNA lesions across the enormous DNA genome remains elusive. It has been hypothesized that glycosylase translocates along the DNA by alternating between a fast but low-accuracy diffusion mode and a slow but high-accuracy mode when searching for DNA lesions. However, the slow mode has not been successfully characterized due to the limitation in the spatial and temporal resolutions of current experimental techniques. Using a newly developed scanning fluorescence resonance energy transfer (FRET)-fluorescence correlation spectroscopy (FCS) platform, we were able to observe both slow and fast modes of glycosylase AlkD translocat

SUBMITTER: Peng S 

PROVIDER: S-EPMC7486748 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature