Hyperpolarized [1-¹³C]pyruvate-to-[1-¹³C]lactate conversion is rate-limited by monocarboxylate transporter-1 in the plasma membrane.

ABSTRACT: Hyperpolarized [1-¹³C]pyruvate magnetic resonance spectroscopic imaging (MRSI) is a noninvasive metabolic-imaging modality that probes carbon flux in tissues and infers the state of metabolic reprograming in tumors. Prevailing models attribute elevated hyperpolarized [1-¹³C]pyruvate-to-[1-¹³C]lactate conversion rates in aggressive tumors to enhanced glycolytic flux and lactate dehydrogenase A (LDHA) activity (Warburg effect). By contrast, we find by cross-sectional analysis using genetic and pharmacological tools in mechanistic studies applied to well-defined genetically engineered cell lines and tumors that initial hyperpolarized [1-¹³C]pyruvate-to-[1-¹³C]lactate conversion rates as well as global conversion were highly dependent on a

SUBMITTER: Rao Y 

PROVIDER: S-EPMC7486767 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature