Three-dimensional alignment of microvasculature and cardiomyocytes in the developing ventricle.

ABSTRACT: While major coronary artery development and pathologies affecting them have been extensively studied, understanding the development and organization of the coronary microvasculature beyond the earliest developmental stages requires new tools. Without techniques to image the coronary microvasculature over the whole heart, it is likely we are underestimating the microvasculature's impact on normal development and diseases. We present a new imaging and analysis toolset to visualize the coronary microvasculature in intact embryonic hearts and quantify vessel organization. The fluorescent dyes DiI and DAPI were used to stain the coronary vasculature and cardiomyocyte nuclei in quail embryo hearts during rapid growth and morphogenesis of the left ventricular wall. Vessel and cardiomyocytes orien

SUBMITTER: Lapierre-Landry M 

PROVIDER: S-EPMC7486945 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature