Efficiency of skeletal muscle decellularization methods and their effects on the extracellular matrix.

ABSTRACT: Extracellular matrix (ECM) is widely considered to be integral to the function of skeletal muscle, providing mechanical support, transmitting force, and contributing to passive stiffness. Many functions and dysfunctions attributed to ECM are thought to stem from its mechanical properties, yet there are few data describing the mechanics of intact ECM. Such measurements require isolating intact ECM from the muscle cells it surrounds. The objectives of this study were to quantify the efficiency of three techniques for this purpose: Triton, Triton with sodium dodecyl sulfate, and latrunculin B; and to determine their impact on properties of the remaining ECM. Efficiency was quantified by DNA content and evaluation of western blot intensities for myosin and actin. The properties of ECM were qua

SUBMITTER: Reyna WE 

PROVIDER: S-EPMC7487037 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature