Dendritic Cells Transfected with MHC Antigenic Determinants of CBA Mice Induce Antigen-Specific Tolerance in C57Bl/6 Mice.

ABSTRACT:

Background

Nonspecific immunosuppressive therapy for graft rejection and graft-versus-host disease (GVHD) is often accompanied by severe side effects such as opportunistic infections and cancers. Several approaches have been developed to suppress transplantation reactions using tolerogenic cells, including induction of FoxP3⁺ Tregs with antigen-loaded dendritic cells (DCs) and induction of CD4⁺IL-10⁺ cells with interleukin IL-10-producing DCs. Here, we assessed the effectiveness of both approaches in the suppression of graft rejection and GVHD.

Methods

IL-10-producing DCs were generated by the transfection of DCs with DNA constructs encoding mouse IL-10. Antigen-loaded DCs from C57BL/6 mice were generated by transfection with DNA constructs e

SUBMITTER: Sennikov SV 

PROVIDER: S-EPMC7487104 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature