Molecular Mechanism Underlying the Action of Zona-pellucida Glycoproteins on Mouse Sperm.

ABSTRACT: Mammalian oocytes are enveloped by the zona pellucida (ZP), an extracellular matrix of glycoproteins. In sperm, stimulation with ZP proteins evokes a rapid Ca²⁺ influx via the sperm-specific, pH-sensitive Ca²⁺ channel CatSper. However, the physiological role and molecular mechanisms underlying ZP-dependent activation of CatSper are unknown. Here, we delineate the sequence of ZP-signaling events in mouse sperm. We show that ZP proteins evoke a rapid intracellular pH _i increase that rests predominantly on Na⁺/H⁺ exchange by NHA1 and requires cAMP synthesis by the soluble adenylyl cyclase sAC as well as a sufficiently negative membrane potential set by the spem-specific K⁺ channel Slo3. The alkaline-activated CatSper cha

SUBMITTER: Balbach M 

PROVIDER: S-EPMC7487327 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature