Meta-transcriptomic identification of Trypanosoma spp. in native wildlife species from Australia.

ABSTRACT:

Background

Wildlife species carry a remarkable diversity of trypanosomes. The detection of trypanosome infection in native Australian fauna is central to understanding their diversity and host-parasite associations. The implementation of total RNA sequencing (meta-transcriptomics) in trypanosome surveillance and diagnosis provides a powerful methodological approach to better understand the host species distribution of this important group of parasites.

Methods

We implemented a meta-transcriptomic approach to detect trypanosomes in a variety of tissues (brain, liver, lung, skin, gonads) sampled from native Australian wildlife, comprising four marsupials (koala, Phascolarctos cinereus; southern brown bandicoot, Isoodon obesulus; swamp wallaby, Wallabia bicolor; bare-nosed womb

SUBMITTER: Ortiz-Baez AS 

PROVIDER: S-EPMC7487544 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature