Alpha-1 antitrypsin deficient individuals have circulating extracellular vesicles with profibrogenic cargo.

ABSTRACT:

Background

Alpha-1 antitrypsin deficiency (AATD)-mediated liver disease is a toxic "gain-of-function" inflammation in the liver associated with intracellular retention of mutant alpha-1 antitrypsin. The clinical presentation of the disease includes fibrosis, cirrhosis and liver failure. However, the pathogenic mechanism of AATD-mediated liver disease is not well understood. Here, we investigated the role of plasma extracellular vesicles (EVs) in progression of AATD-mediated liver disease.

Methods

EVs were isolated from plasma of AATD individuals with liver disease and healthy controls. Their cytokines and miRNA content were examined by multiplex assay and small RNA sequencing. The bioactivity of EVs was assessed by qPCR, western blot analysis and immunofluorescent experiment

SUBMITTER: Khodayari N 

PROVIDER: S-EPMC7487708 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature