Leishmania infection induces a limited differential gene expression in the sand fly midgut.

ABSTRACT:

Background

Sand flies are the vectors of Leishmania parasites. To develop in the sand fly midgut, Leishmania multiplies and undergoes various stage differentiations giving rise to the infective form, the metacyclic promastigotes. To determine the changes in sand fly midgut gene expression caused by the presence of Leishmania, we performed RNA-Seq of uninfected and Leishmania infantum-infected Lutzomyia longipalpis midguts from seven different libraries corresponding to time points which cover the various Leishmania developmental stages.

Results

The combined transcriptomes resulted in the de novo assembly of 13,841 sand fly midgut transcripts. Importantly, only 113 sand fly transcripts, about 1%, were differentially expressed in the presence of Leishmania parasites. Further,

SUBMITTER: Coutinho-Abreu IV 

PROVIDER: S-EPMC7487717 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature