Plasma proteins facilitates placental transfer of polystyrene particles.

ABSTRACT:

Background

Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this study we were focused on the impact of in situ formed human plasma protein corona on the transfer of 80 nm polystyrene nanoparticles (PS-particles) across the human placenta. To study materno-to fetal PS transfer we used the human ex vivo placental perfusion approach, which represents an intact and physiological tissue barrier. To analyze the protein corona of PS particles we performed shotgun proteomics of isolated nanoparticles before and after tissue exposure.

Results

Human plasma incuba

SUBMITTER: Gruber MM 

PROVIDER: S-EPMC7487953 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature