Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus.

ABSTRACT:

Background

As a type of high-frequency electrotherapy, a short-wave can promote the fracture healing process; yet, its underlying therapeutic mechanisms remain unclear.

Purpose

To observe the effect of Short-Wave therapy on mesenchymal stem cell (MSC) homing and relative mechanisms associated with fracture healing.

Materials and methods

For in vivo study, the effect of Short-Wave therapy to fracture healing was examined in a stabilized femur fracture model of 40 SD rats. Radiography was used to analyze the morphology and microarchitecture of the callus. Additionally, fluorescence assays were used to analyze the GFP-labeled MSC homing after treatment in 20 nude mice with a femoral fracture. For in vitro study, osteoblast from newborn rats simulated fracture site was fi

SUBMITTER: Ye D 

PROVIDER: S-EPMC7487968 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature