High sensitivity of one-step real-time reverse transcription quantitative PCR to detect low virus titers in large mosquito pools.

ABSTRACT:

Background

Mosquitoes are the deadliest animals in the world. Their ability to carry and spread diseases to humans causes millions of deaths every year. Due to the lack of efficient vaccines, the control of mosquito-borne diseases primarily relies on the management of the vector. Traditional control methods are insufficient to control mosquito populations. The sterile insect technique (SIT) is an additional control method that can be combined with other control tactics to suppress specific mosquito populations. The SIT requires the mass-rearing and release of sterile males with the aim to induce sterility in the wild female population. Samples collected from the environment for laboratory colonization, as well as the released males, should be free from mosquito-borne viruses (MBV).

SUBMITTER: Tang Z 

PROVIDER: S-EPMC7488135 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature