Genistein contributes to cell cycle progression and regulates oxidative stress in primary culture of osteoblasts along with osteoclasts attenuation.

ABSTRACT:

Background

The present study was designed to examine the role of isoflavone genistein (GS) on bone formation, regulating oxidative stress and cell cycle in primary osteoblasts, as well as attenuation of osteoclast formation.

Methods

Primary calvaria osteoblasts were isolated from 2 to 3 days old neonatal rat pups (n = 6-8) of Sprague Dawley rats. Osteoblasts were incubated with varying concentrations of GS and different assays viz. cell proliferation, differentiation, calcium deposition, cell cycle progression, antioxidant ability, and osteogenic gene expression were performed. Tartrate-resistant acid phosphatase (TRAP) staining and immunolocalization of cathepsin K protein were assessed in bone marrow-derived osteoclasts.

Results

Results revealed that GS markedly ind

SUBMITTER: Siddiqui S 

PROVIDER: S-EPMC7488498 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature