Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study.

ABSTRACT:

Background

Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (iPSCs) hold great promise in toxicological applications as well as in regenerative medicine. Previous efforts on hepatocyte differentiation have mostly relied on the use of growth factors (GFs) to recapitulate developmental signals under in vitro conditions. Recently, the use of small molecules (SMs) has emerged as an attractive tool to induce cell fate transition due to its superiority in terms of both quality and cost. However, HLCs derived using SMs have not been well characterized, especially on the transcriptome level.

Methods

HLCs were differentiated from human iPSCs using a protocol that only involves SMs and characterized by transcriptomic analysis using whole genome microarray

SUBMITTER: Gao X 

PROVIDER: S-EPMC7488531 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature