Mechanical force-induced morphology changes in a human fungal pathogen.

ABSTRACT:

Background

The initial step of a number of human or plant fungal infections requires active penetration of host tissue. For example, active penetration of intestinal epithelia by Candida albicans is critical for dissemination from the gut into the bloodstream. However, little is known about how this fungal pathogen copes with resistive forces upon host cell invasion.

Results

In the present study, we have used PDMS micro-fabrication to probe the ability of filamentous C. albicans cells to penetrate and grow invasively in substrates of different stiffness. We show that there is a threshold for penetration that corresponds to a stiffness of ~ 200 kPa and that invasive growth within a stiff substrate is characterized by dramatic filament buckling, along with a stiffness-dependen

SUBMITTER: Puerner C 

PROVIDER: S-EPMC7488538 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature