Hippocampal interleukin-33 mediates neuroinflammation-induced cognitive impairments.

ABSTRACT:

Background

Interleukin (IL)-33 is expressed in a healthy brain and plays a pivotal role in several neuropathologies, as protective or contributing to the development of cerebral diseases associated with cognitive impairments. However, the role of IL-33 in the brain is poorly understood, raising the question of its involvement in immunoregulatory mechanisms.

Methods

We administered recombinant IL-33 (rmIL-33) by intra-hippocampal injection to C57BL/6 J (WT) and IL-1αβ deficient mice. Chronic minocycline administration was performed and cognitive functions were examined trough spatial habituation test. Hippocampal inflammatory responses were investigated by RT-qPCR. The microglia activation was assessed using immunohistological staining and fluorescence-activated cell sorting

SUBMITTER: Reverchon F 

PROVIDER: S-EPMC7488545 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature